anti-CD14 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD14; anti-CD14 antibody;
The CD14 cd14 (Catalog #MBS8510527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD14, Purified mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD14 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD14 cd14 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD14 cd14 product has the following accession number(s) (GI #2274810) (NCBI Accession #BAA21517.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse anti-Human CD14, Purified mAb with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Human peripheral blood granulocytes analyzed with Purified CD14 mAb, followed by anti-mouse IgGs-PE)
W4 clone reacts with CD14, a 53-55 kDa molecule. CD14 is a human high affinity cell-surface receptor for complexes of lipopolysaccharide (LPS-endotoxin) and serum LPS-binding protein (LPB). CD14 antigen has a strong presence on the surface of monocytes/macrophages, is weakly expressed on granulocytes, but not expressed by myeloid progenitor cells. CD14 functions as a receptor for endotoxin; when the monocytes become activated they release cytokines such as TNF, and up-regulate cell surface molecules including adhesion molecules. This clone is cross reactive with non-human primate.
Experimental Methods: 1. Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2. Add appropriate amount of antibody to the bottom of flow tube mixing with the whole blood, incubate for 30 minutes at room temperature;
3. Add 2 ml1�RBC lysis buffer, incubate for 10 minutes after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10�);
4. Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5. Add 2 ml PBS wash buffer to resuspend the cells, then 1000 rpm centrifugation for 5 minutes, discard the supernatant;
6. Add appropriate amount of fluorescent-labeled anti-mouse IgGs and incubate for 20 minutes away from light at room temperature.
7. Repeat step 5.
8. Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD14 are readily searchable from our website. Different antibodies against the same target such as CD14 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.