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anti-CD147 antibody product blog

Posted on 2016-01-24 05:00:16 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD147; anti-CD147 antibody;
The CD147 bsg (Catalog #MBS520056) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD147, Purified (Clone OX114) (rat IgG1) reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD147 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC).
This antibody is suitable for use in flow cytometry, Western Blotting and immunohistochemistry. Researchers should empirically determine the suitability of the CD147 bsg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD147 bsg product has the following accession number(s) (GI #31076333) (NCBI Accession #BAC76828.1) (Uniprot Accession #P35613). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Mouse CD147, Purified (Clone OX114) (rat IgG1) with the following immunoassay(s):
Testing Data
Testing Data CD147.

CD147 is a widely expressed membrane glycoprotein (also known as OX47, basigin, EMMPRIN and HT7; expressed on leukocytes, red cells, platelets and endothelial cells) and has been implicated in matrix metalloproteinase induction, cell adhesion, retinal cell development and T cell activation. The transmembrane region has a very high degree of homology between various species, and is identical between chicken and rat. anti-mouse CD147 antibody detects both the two and three Ig-like domain isoforms of the CD147 extracellular region on the surface of transfected HEK293T cells. In Western Blotting, these solubilized cell lysates showed bands at 38 and 50 kDa for domains two and three respectively. This antibody also gave good staining of mouse thymocytes and fixed tissue sections. Only the three domain form interacts homophilically, and this form has only been found in the retina and not in other tissues (ie. brain, lung, liver, spleen). The two domain form associates laterally with the monocarboxylate transport proteins MCT1 and MCT4.

Immunogen: Recombinant md0-2rCD4d3+4
Donor: rat spleen
Fusion Partner: Y3 myeloma cells. Presentation: HPurified IgG buffered in PBS and 0.02% NaN3. (Purified from ascitic fluid via Protein G Chromatography). For maximum recovery of contents, spin down tube before use. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD147 are readily searchable from our website. Different antibodies against the same target such as CD147 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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