anti-CD154 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD154; anti-CD154 antibody;
The CD154 n/a (Catalog #MBS666518) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD154-Purified (with Antibiotics) reacts with Human, Primate and may cross-react with other species as described in the data sheet. MyBioSource\'s CD154 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Western Blot (WB), Immunohistchemistry (IHC). Researchers should empirically determine the suitability of the CD154 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD154-Purified (with Antibiotics) with the following immunoassay(s):
Testing Data
Information: Human CD154 (CD40 Ligand) is a member of the tumor necrosis factor (TNF) family and is expressed on the surface of activated T cells. It can undergo proteolytic cleavage into an immunologically active soluble form. Interaction of CD154 and CD40 is essential for isotype switching in B cells. Known genetic defects that alter this interaction lead to impaired immune system function (1). Increased levels of CD154 has been associated with autoimmune disorders including SLE, CLL and eosinophilic fasciitis (5,9,10,11). CD154 has been reported to be expressed on vascular endothelial cells, smooth muscle cells, macrophages and activated platelets indicating a role for the CD40-CD154 immunoregulatory signaling in artherosclerosis and cardiovascular disorders (7,12,13). Antibody 24-31 immunoprecipitates a CD154 (gp39) molecule of about 39 kd. The antibody 24-31 will block MLR, sgp39 induced human B cell proliferation and T cell dependent B cell differentiation.
Performance: Five x 105 Jurkat-4G (a CD154+ subclone of Jurkat) cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-CD154 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.67 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD154
Immunogen: Human sgp39 fusion protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD154 are readily searchable from our website. Different antibodies against the same target such as CD154 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.