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anti-CD16/32 antibody product blog

Posted on 2016-01-31 13:16:09 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD16/32; anti-CD16/32 antibody;
The CD16/32 n/a (Catalog #MBS833913) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD16/32 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC), Immunoprecipitation (IP), Western Blot (WB).
FC: <= 2 ug/test

A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD16/32 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD16/32 antibody (allophycocyanin) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of BALB/c splenocytes with staining buffer (Autofluorescence) (open histogram) or 0.125 ug of CD16/CD32 antibody (filled histogram). Total viable cells were used for analysis.)
Flow Cytometry (FC/FACS) CD16/32.

Rat monoclonal CD16/32 antibody (allophycocyanin).

Biological Significance: The 93 monoclonal antibody reacts with an epitope shared by mouse CD16 and CD32. CD16 and CD32 are the low affinity receptors for the mouse IgG Fc portion and are expressed by B cells, monocyte/macrophages, NK cells, and neutrophils. Tag/Conjugate: Allophycocyanin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD16/32 are readily searchable from our website. Different antibodies against the same target such as CD16/32 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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