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anti-CD16/CD32 antibody product blog

Posted on 2016-03-15 17:41:43 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD16/CD32; anti-CD16/CD32 antibody;
The CD16/CD32 n/a (Catalog #MBS2534376) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse CD16/CD32 Antibody (BIOT conjugated) reacts with Mouse and may cross-react with other species as described in the data sheet.
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used <= 0.5 ug /106 cells in 100 ul). Since applications vary, the appropriate dilutions must be determined for individual use. Researchers should empirically determine the suitability of the CD16/CD32 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Anti-Mouse CD16/CD32 Antibody (BIOT conjugated) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (C57BL/6 mouse splenocytes stained with Biotin anti-mouse CD16/32, followed by PE-SA)
Flow Cytometry (FC/FACS) CD16/CD32.

The 2.4G2 antibody reacts specifically with a common nonpolymorphic epitope on the extracellular domains of the mouse FcgammaIII and FcgammaII receptors. It has also been reported to bind the FcgammaI receptor (CD64) via its Fc domain. 2.4G2 mAb blocks non-antigen-specific binding of immunoglobulins to the FcgammaIII and FcgammaII, and possibly FcgammaI, receptors in vitro and in vivo. CD16 and/or CD32 are expressed on natural killer cells, monocytes, macrophages, dendritic cells (at low levels), Kupffer cells, granulocytes, mast cells, B lymphocytes, immature thymocytes, and some activated mature T lymphocytes.

Conjugation: BIOT. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD16/CD32 are readily searchable from our website. Different antibodies against the same target such as CD16/CD32 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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