anti-CD163 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD163; anti-CD163 antibody;
The CD163 cd163 (Catalog #MBS249224) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 3B4] (IgG2a) to Human CD163 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD163 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB).IF (1:100), IHC-P (10 ug/ml), WB (1:200 - 1:4000). Researchers should empirically determine the suitability of the CD163 cd163 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD163 cd163 product has the following accession number(s) (GI #44662834) (NCBI Accession #NP_004235.3) (Uniprot Accession #Q86VB7). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 3B4] (IgG2a) to Human CD163 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CD163 antibody IHC staining of human liver. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 10 ug/ml.)
Immunofluorescence (IF) (Anti-CD163 mouse monoclonal antibody immunofluorescent staining of COS7 cells transiently transfected by pCMV6-ENTRY CD163.)
Western Blot (WB) (Western blot of extracts (35 ug) from 9 different cell lines by using anti-CD163 monoclonal antibody (HepG2: human; HeLa: human; SVT2: mouse; A549: human; COS7: monkey; Jurkat: human; MDCK: canine; PC12: rat; MCF7: human).)
Western Blot (WB) (HEK293T cells were transfected with the pCMV6-ENTRY control (Left lane) or pCMV6-ENTRY CD163 (Right lane) cDNA for 48 hrs and lysed. Equivalent amounts of cell lysates (5 ug per lane) were separated by SDS-PAGE and immunoblotted with anti-CD163.)
Target Species: Human
Immunogen Description: Full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell. Immunogen Type: Recombinant protein
Immunogen: CD163 antibody was raised against full length human recombinant protein of human CD163(NP_004235) produced in HEK293T cell. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD163 are readily searchable from our website. Different antibodies against the same target such as CD163 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene.