|The CD166 alcam (Catalog #MBS584070) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD166, Human, mAb AZN-L50 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD166 can be used in a range of immunoassay formats including, but not limited to, F, Flow Cytometry (FC/FACS), FS, Immunoflurescence (IF), Immunoprecipitation (IP), P, Western Blot (WB).
Application Notes: IHC-F:- Tissue sections were fixed in acetone
Application Use: For immunohistology, flow cytometry and Western blotting, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. For functional studies, in vitro dilutions have to be optimized in user’s experimental setting. Researchers should empirically determine the suitability of the CD166 alcam for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD166 alcam product has the following accession number(s) (GI #343168776) (NCBI Accession #NP_001230212.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Product Description: The monoclonal antibody AZN-L50 recognizes activated leukocyte cell adhesion molecule (ALCAM), a member of the immunoglobulin superfamily and has a characteristic VVC2C2C2 domain structure. ALCAM is a type I transmembrane protein of 100 kDa with five extracellular immunoglobulin-like domains and a short cytoplasmic tail. ALCAM mediates homotypic ALCAM-ALCAM interactions and heterotypic interactions with the T-cell antigen CD6. Homotypic interactions are involved in the development and maintenance of tissue architecture and tumor progression. Heterotypic interactions initiate and stabilize T-cell-dendritic interactions affecting T-cell activation.
ALCAM is secreted as a NH2-terminal fragment of 30 kD. Soluble ALCAM (sALCAM) induces divergent biological signals and responses.
ALCAM shows a characteristic, temporal and spatial distribution in development of a wide variety of tissues and cells in health and disease. ALCAM is expressed on a wide variety of cells, within the leukocyte population its expression is particularly high on dentritic cells. ALCAM is involved in various physiological processes including hematopoieses, thymus development, the immune response, neurite extension, neural cell migration, embryogenesis, neurogenesis, angiogenesis, osteogenesis and is a marker for pluripotent mesenchymal stem cells.
The monoclonal antibody AZN-L50 completely blocks homophilic ALCAM-ALCAM interaction.
Immunogen: CD166 expressing K562 cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD166 are readily searchable from our website. Different antibodies against the same target such as CD166 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.