anti-CD172a antibody product blog
Tags: Antibody; Monoclonal Antibody; CD172a; anti-CD172a antibody;
The CD172a n/a (Catalog #MBS832885) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD172a can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 0.06 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD172a n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD172a antibody (allophycocyanin) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with Mouse IgG2a K Isotype Control (left) or CD172a antibody (right). Cells in the lymphocyte (green histogram) or granulocyte (blue histogram) gate were used for analysis.)
Mouse monoclonal CD172a antibody (allophycocyanin).
Biological Significance: This 15-414 monoclonal antibody reacts with human CD172a, also known as signal regulatory protein alpha. This cell surface glycoprotein consists of three Ig-like extracellular domains and two cytoplasmic immunoreceptor tyrosine-based inhibitory motifs. The ITIM domains have been demonstrated to recruit and bind the Src homology 2 domain-containing phosphatases SHP-1 and SHP-2. CD172a is expressed on monocytes, granulocytes, dendritic cells, and CD34CD38- hematopoietic progenitor cells but not on T and B lymphocytes. Moreover, neurons have also been shown to express CD172. The integrin-associated protein CD47 is the extracellular ligand for CD172a. Studies show that CD172a is involved in dendritic cell-mediated T cell activation, neutrophil migration, and phagocytosis. Tag/Conjugate: Allophycocyanin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD172a are readily searchable from our website. Different antibodies against the same target such as CD172a may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.