anti-CD178 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD178; anti-CD178 antibody;
The CD178 faslg (Catalog #MBS212781) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI HUMAN CD178 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD178 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Flow cytometry (FC/FACS), Immunoprecipitation (IP).Flow Cytometry:
- Suggested Dilution: 1/25 - 1/50
- Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
- Results maybe enhanced using membrane permeabilization. The use of Leucoperm™ (Please Inquire) prior to staining is recommended.
Where this antibody has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the antibody for use in their own system using appropriate negative/positive controls. Researchers should empirically determine the suitability of the CD178 faslg for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD178 faslg product has the following accession number(s) (GI #4557329) (NCBI Accession #NP_000630.1) (Uniprot Accession #P48023). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD178 with the following immunoassay(s):
Testing Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178 (MBS212780))
Testing Data (Staining of CD178 transfected CHO cells with Mouse anti Human CD178: Alexa Fluor 647 (MBS212780A647))
Testing Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178)
Testing Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178:Alexa Fluor 488 (MBS212780A488))
Testing Data (Staining of permeabilized Jurkat cells with Mouse anti Human CD178:RPE (MBS213807))
Testing Data (Sandwich ELISA analysis of CD178 binding using Mouse anti Human CD178 (MBS212780) as a capture reagent and biotinylated Mouse anti Human CD178 (MBS212778) as a detection reagent with purified human CD178 as antigen for the generation of a standard curve. Detection is by HRP conjugated Streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. Plasma (orange) sample is displayed at 1:2 dilution.)
Preservative Stabilizers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human
Carrier Free: Yes
Fusion Partners: Spleen cells from immunized BALB/c mice were fused with cells of the P3U1 myeloma cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD178 are readily searchable from our website. Different antibodies against the same target such as CD178 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.