anti-CD19 antibody product blog
Tags: Antibody; anti-CD19 antibody; CD19; Monoclonal Antibody;
The CD19 cd19 (Catalog #MBS666360) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD19-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD19 cd19 product has the following accession number(s) (GI #4105281) (NCBI Accession #AAD02340.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD19-Biotin with the following immunoassay(s):
Testing Data
Information: Human CD19 is a B cell restricted membrane protein expressed from early stages of B cell development and associates with the antigen receptor complex. CD19 is involved in the regulation of B cell proliferation but eventually disappears from plasma cells. Antibody BU12 recognizes the CD19 molecule of about 95 kd. Antibody BU12 induces adhesion of B cells.
Performance: Five x 105 cultured Raji cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD19/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.19 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with a 10-fold excess of unlabeled anti-CD19 antibody. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human EB-4 Burkitt lymphoma cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD19 are readily searchable from our website. Different antibodies against the same target such as CD19 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Asthma, Cardiovascular Diseases, Cell Transformation, Neoplastic, Death, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Respiratory Tract Diseases, Respiratory Tract Infections are some of the diseases may be linked to anti-human CD19-Biotin. Blood, Bone Marrow, Brain, Intestine, Lung, Lymph, Lymph Node, Spleen, Thymus, Tonsil tissues are correlated with this protein. CD19 also interacts with the following gene(s): BTK, CD22, CD79A, CD79B, CD81, CR2, LYN, SYK, VAV1. The following patways have been known to be associated with this gene.