|The CD19 cd19 (Catalog #MBS668356) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to hCD19 (Clone: CB19) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD19 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB).
WB: 4-6 ug/ml
Flow Cytometry: 0.5-1 ug/10^6. Researchers should empirically determine the suitability of the CD19 cd19 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD19 cd19 product has the following accession number(s) (GI #160332376) (NCBI Accession #P15391.6) (Uniprot Accession #P15391). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to hCD19 (Clone: CB19) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Fig-1: Cell Surface flow staining of hCD19 in PBMCs (lymphocytes gated) using 0.5 ug antibody per 10^6 cells of antibody (Clone: CB19). Green represents isotype control ; red represents anti-hCD19 antibody. Goat anti-mouse PE conjugate secondary antibody was used.)
Flow Cytometry (FC/FACS) (Fig-2: Surface flow cytometric analysis of CD19 on Raji cells using 0.5 ug antibody per 10^6 cells of antibody (Clone: CB19). Green represents isotype control; red represents anti-CD19 antibody. Goat anti-mouse PE conjugate secondary antibody was used.)
Western Blot (WB) (Fig-3: Western blot analysis of hCD19. Anti-hCD19 antibody (Clone: CB19) was used at 2 ug/ml on Daudi lysate.)
Immunogen Information: Intact normal human B cell cells were used as immunogen for this antibody. Antigen: hCD19. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD19 are readily searchable from our website. Different antibodies against the same target such as CD19 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CD19 also interacts with the following gene(s): BTK, CD22, CD79A, CD79B, CD81, CR2, LYN, SYK, VAV1. The following patways have been known to be associated with this gene. Blood, Bone Marrow, Brain, Intestine, Lung, Lymph, Lymph Node, Spleen, Thymus, Tonsil tissues are correlated with this protein. Asthma, Cardiovascular Diseases, Cell Transformation, Neoplastic, Death, Inflammation, Liver Diseases, Lung Diseases, Necrosis, Respiratory Tract Diseases, Respiratory Tract Infections are some of the diseases may be linked to Monoclonal Antibody to hCD19 (Clone: CB19).