|The CD1d cd1d1 (Catalog #MBS224527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI RAT CD1d reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD1d can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP), Western Blot (WB).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul.
Flow Cytometry: Maximum Dilution: 1/2,000. Researchers should empirically determine the suitability of the CD1d cd1d1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD1d cd1d1 product has the following accession number(s) (GI #161484595) (NCBI Accession #NP_031665.2). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD1d with the following immunoassay(s):
Testing Data #1 (Staining of rat thymocytes with Mouse anti Rat CD1d (MBS224527))
Mouse anti Rat CD1d antibody, clone WTH2 recognizes rat CD1d, a glycoprotein expressed on cortical thymocytes, certain T-cell leukaemias and various other antigen presenting cells. CD1d binds self and non-self glycolipids and presents them to T-cell receptors on natural killer cells. When activated, natural killer cells produce Th1 and Th2 cytokines. Clone WTH2 recognizes a different, non-overlapping epitope than clone WTH1 and binds with high affinity to the mature CD1d heavy chain independently of beta-2-microglobulin. Mouse anti Rat CD1d antibody, clone WTH2 efficiently blocks rat and mouse invariant natural killer T cell (iNKT cell) response to a-GalCer (an antigen for iNKT cells), consistently blocks activation of mouse type II NKT cell hybridomas and has a slightly lower affinity to native CD1d than clone WTH1. In western blotting clone WTH2 reacts weakly with rat tissue and does not cross react with mouse tissue. It reacts strongly with both rat and mouse antigens in flow cytometry and cross reacts weakly with mouse tissue in IHC and immunoprecipitation.
Perservative Stabilisers: 0.02% Sodium Azide (NaN3). Immunogen: Rat CD1d-transduced M12.4.1.C3 cells.
Buffer Solution: Phosphate buffered saline
Target Species: Rat. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD1d are readily searchable from our website. Different antibodies against the same target such as CD1d may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.