anti-CD200R3 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD200R3; anti-Cd200r3 antibody;
The CD200R3 cd200r3 (Catalog #MBS584690) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The CD200R3, Mouse, mAb Ba91, FITC reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD200R3 can be used in a range of immunoassay formats including, but not limited to, Flow Ctometry (FC/FACS).For flow cytometry, dilutions to be used depend on detection system applied. It is recommended that users test the reagent and determine their own optimal dilutions. The typical starting working dilution is 1:50. Researchers should empirically determine the suitability of the CD200R3 cd200r3 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD200R3 cd200r3 product has the following accession number(s) (GI #189409166) (NCBI Accession #NP_001121605.1) (Uniprot Accession #Q5UKY4). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The monoclonal antibody Ba91 recognizes mouse CD200 Receptor-like 3 (CD200R3), a protein of ~38 kDa or ~74 kDa (reduced or non-reduced condition, respectively). CD200R3 presumably exists as a disulfide-linked dimer on the cell surface. The OX-2, or CD200 receptor (CD200R), belongs to the immunoglobulin superfamily. CD200R contains two Ig-like extracellular domains and mediates inhibitory signals in myeloid cells. Mice deficient for the ligand CD200 develop enhanced experimental allergic encephalomyelitis and collagen-induced arthritis. Unlike CD200R, the CD200R-like receptors functions as activating receptors. They contain short cytoplasmic tails and a lysine residue in the transmembrane region and are likely to signal via adaptor proteins such as DAP12, DAP10, FcRg, or CD3z. CD200R3 is expressed preferentially on cells of the myeloid lineage, including mast cells and basophils. Both cell types are involved in the host defense system against pathogens and in the development of allergic disorders. They function as independent essential initiators of allergic reactions. Basophils are the least common leukocytes in the peripheral blood accounting approximately 0.5 % of all leukocytes. In vivo depletion of basophils by using monoclonal antibody proved the essential role of basophils in the development and maintenance of IgE-mediated chronic allergic inflammation. The activating CD200 receptors, like CD200R3, play an important role in IgE-independent mast cell and basophil activation. Monoclonal antibody Ba91 activates basophils ex vivo and elicits systemic anaphylaxis when administered in vivo. Cross-linking of CD200R3 on MC/9 cells by monoclonal antibody Ba91 induces degranulation. Monoclonal antibody Ba91 is also useful for depletion of basophils.