|The CD21 n/a (Catalog #MBS224789) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The MOUSE ANTI BOVINE CD21: RPE reacts with Bovine and may cross-react with other species as described in the data sheet. MyBioSource\'s CD21 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD21 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI BOVINE CD21: RPE with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21:FITC )
Flow Cytometry (FC/FACS) (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21:RPE)
Flow Cytometry (FC/FACS) (Staining of bovine peripheral blood lymphocytes with Mouse anti Bovine CD21 followed by Goat anti Mouse IgG:FITC )
Mouse anti Bovine CD21 monoclonal antibody, clone CC51 recognizes the bovine homologue of the human CD21 cell surface antigen, a 145 kDa single pass type I membrane glycoprotein containing multiple sushi domains. CD21 is also known as complement receptor type 2 (CR2). In cattle CD21 expression is restricted to B-cells (Naessens et al. 1990). CD21 may be expressed on B-cells as either a long or a short form (Pringle et al. 2012). Mouse anti Bovine CD21, clone CC51 demonstrates cross reactivity with porcine and provides a reliable marker for porcine B-Cells (Sinkora et. al. 2013). In addition to clone CC51, clone CC21 has been demonstrated to recognise CD21 in a range of ruminant and other species.
Reactivity Notes: African Buffalo, Pig
Preparation: Purified IgG prepared by affinity chromatography on Protein A
Preservative Stabilisers: 0.09% Sodium Azide (NaN3), 1% Bovine Serum Albumin, 5% Sucrose
Buffer Solution: Phosphate buffered saline. Reconstitution: Reconstitute with 1.0 ml distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. We recommend that the vial is gently mixed after reconstitution. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD21 are readily searchable from our website. Different antibodies against the same target such as CD21 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.