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anti-CD23 antibody product blog

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Posted on 2016-01-10 10:38:16 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD23; anti-CD23 antibody;
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The CD23 fcer2 (Catalog #MBS213794) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD23 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat; Maximum Dilution: 1/5. Researchers should empirically determine the suitability of the CD23 fcer2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD23 fcer2 product has the following accession number(s) (GI #359339166) (NCBI Accession #NP_001240666.1) (Uniprot Accession #P20693). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD23:RPE with the following immunoassay(s):
Testing Data (Staining of mouse spleen cells with Rat anti Mouse CD23: Alexa Fluor 647 (MBS212722A647))
Testing Data CD23.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD23 (MBS212722))
Testing Data CD23.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD23: Alexa Fluor 488 (MBS212722A488))
Testing Data CD23.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD23:RPE)
Testing Data CD23.

Rat anti Mouse CD23 antibody, clone B3B4 recognizes murine CD23, the ~49kDa low affinity Fc receptor for IgE (Fc epsilon RII). In the mouse, CD23 is primarily expressed on mature B lymphocytes and follicular dendritic cells. CD23 is known to play an important role in IgE binding but reports suggest that CD23 may also be involved in B-cell proliferation and differentiation. Rat anti Mouse CD23 antibody, clone B3B4 is reported to block the binding of IgE to Fc epsilon RII (Rao, M. et al.).

Reconstitution: Reconstitute with 1.0 ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
5% Sucrose
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD23 are readily searchable from our website. Different antibodies against the same target such as CD23 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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