anti-CD23 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD23; anti-CD23 antibody;
The CD23 fcer2 (Catalog #MBS570329) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti CD23, Low affinity immunoglobulin epsilon Fc receptor reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD23 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).The 5E3 antibody is suitable for the detection of human CD23 antigen by flow cytometry. Human tonsil is suitable as a positive control.
Optimal antibody dilutions for the different applications should be determined by titration. Researchers should empirically determine the suitability of the CD23 fcer2 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD23 fcer2 product has the following accession number(s) (GI #334085205) (NCBI Accession #NP_001193948.2) (Uniprot Accession #P06734). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
The low affinity immunoglobulin E (IgE) Fc receptor, also known as CD23, is a 45kD type II transmembrane glycoprotein. CD23 contains a C-type lectin domain in the extracellular C-terminal region and belongs to group II of the type II lectins. CD23 is a receptor for IgE but can also bind to other ligands such as CD21, CD11b and CD11c. CD23 primarily functions as a B-cell growth and activation factor. It also regulates the synthesis of IgE through binding of IgE and CD21. Two isoforms of CD23 have been described, CD23a and CD23b, which are expressed on different cell types. CD23a expression is restricted to resting B-cells, whereas CD23b is weakly expressed on activated B-cells and other haematopoietic cells. CD23 is also expressed on B-cell chronic lymphocytic leukemia (CLL) cells and is useful as a marker to differentiate CLL from mantle cell lymphoma by flow cytometry. A soluble form of CD23 (sCD23) also exists which is produced by proteolytic cleavage of the membrane bound form. Studies suggest that levels of serum sCD23, in cases of B-CLL, correlate with tumour activity and are an important indicator for early treatment.
Source Note: 5E3 is a mouse monoclonal IgG1 antibody derived by fusion of SP2/0 mouse myeloma cells with splenocytes from a BALB/c mouse immunized with Daudi cells, a human Burkitt\'s lymphoma cell line. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD23 are readily searchable from our website. Different antibodies against the same target such as CD23 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.