anti-CD25 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD25; anti-CD25 antibody;
The CD25 il2ra (Catalog #MBS213821) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD25 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD25 il2ra for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD25 il2ra product has the following accession number(s) (GI #7710038) (NCBI Accession #NP_037295.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI RAT CD25:RPE with the following immunoassay(s):
Testing Data (Staining of Con An activated rat spleen cells with Mouse anti Rat CD25: Biotin (MBS210477))
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. Medium power)
Testing Data (Staining of stimulated rat spleen cells with Mouse anti Rat CD25:FITC (MBS216315))
Testing Data (Published clone specific image: The percentage of Ly49s3+ NK cells increases in the spleen and decreases in the bone marrow following L. monocytogenes infection. A) Mononuclear leukocytes were harvested from spleen, blood, and bone marrow from uninfected and infected animals 24 and 48 hrs p.i. Cells were stained with antibodies to NKR-P1A, CD3, and Ly49s3. NK cells were gated as NKR-P1A+CD3- cells, and the percentage of Ly49s3+ NK cells was analysed by flow cytometry. The data represent the mean +/- SEM from three independent experiments, with 2 animals at each time point. Data were analyzed with the one-way ANOVA with Tukey\'s multiple comparisons test. B) The activation status of NK cells from uninfected (filled histogram) or infected animals 24 (grey line) and 48 hrs p.i. (black line) was tested by flow cytometry. An antibody towards the activation marker CD25 was employed.From: Shegarfi H, Naper C, Rolstad B, Inngjerdingen M (2010) Listeria monocytogenes Infection Affects a Subset of Ly49-Expressing NK Cells in the Rat. PLoS ONE 5(12): e15579.)
Testing Data (Staining of rat spleen cells with Mouse anti Rat CD25: FITC (MBS210996))
Testing Data (Published customer image: Analysis of graft-infiltrating T cells. Activated CD25+ T cells and CD4+ and CD8+ T cell subsets were stained at the time points of corneal allograft rejection and calculated within the graft. A, C, and E show representative histological staining for CD25, CD4, and CD8 in grafts of treated and control animals, respectively. CD25+ (B) and CD4+ (D) cells infiltrated to a statistically significantly stronger extent in 3.2.3-treated animals when compared to control treated animals (*p)
Testing Data (Immunofluorescence staining of rat lymph node cryosection with Mouse anti Rat CD25 antibody, red in An and Mouse anti Rat CD8, green in B. C is the merged image with nuclei counterstained blue using DAPI. Medium power)
Testing Data (Immunoperoxidase staining of rat lymph node cryosection with Mouse anti Rat CD25 followed by horseradish peroxidase conjugated Goat anti Mouse IgG1 (MBS235406) as a detection reagent. High power)