anti-CD25 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD25; anti-CD25 antibody;
The CD25 pmp22 (Catalog #MBS666125) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD25-APC reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD25 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunohistochemistry (IHC).1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD25 pmp22 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD25 pmp22 product has the following accession number(s) (GI #55904) (NCBI Accession #CAA44297.1) (Uniprot Accession #P25094). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Information: Expression of human CD25 (the alpha subunit of the IL-2 receptor complex) is dramatically upregulated on activated T cells, B cells, NK cells and macrophages (3). Antibodies to CD25 are being studied for potential use as an immundirected therapy to selectively attack (kill) CD25 expressing cells (4). Antibody 7G7B6 recognizes epitope B of the IL-2 alpha receptor of about 55 kd (1,6). Antibody 7G7B6 does not block IL-2 binding to its receptor (1).
Performance: Cultured human CEM were stimulated overnight in the presence of PMA (10ng/ml) and ionomycin (1uM). Five x 105 cells per tube were incubated 45 minutes on ice with 80 ul of anti-CD25/R-PE at a dilution factor of 1:50 (5 ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.24 log10 fluorescent units when compared to a Mouse IgG1/APC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD25 antibody. Conjugation: APC
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.25 mg/ml with an A650/A280 ratio of 1.48.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Stimulated PBMC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD25 are readily searchable from our website. Different antibodies against the same target such as CD25 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.