|The CD27 cd27 (Catalog #MBS666606) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD27-R-PE reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s CD27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA).
1:50 (or use 1.6ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD27 cd27 product has the following accession number(s) (GI #4507587) (NCBI Accession #NP_001233.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD27-R-PE with the following immunoassay(s):
Information: Human CD27 is a lymphocyte specific member of the tumor necrosis factor (TNF) receptor family and is found primarily on peripheral blood T cells and on a subpopulation of B cells and NK cells. The ligand for CD27 is CD70, which is a member of the TNF ligand superfamily. Antibody M-T271 recognizes the dimeric CD27 molecule of approximately 110 kd.
Performance: Five x 105 ficoll prepared human peripheral blood mononuclear cells were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were washed and incubated 45 minutes on ice with 80 ul of anti-CD27/R-PE at a 1:50 dilution (5 ug/ml). Cells were washed three times, fixed and analyzed by FACS. A net 61% subpopulation of cells stained positive with a mean shift of 1.97 log10 fluorescent units when compared to a Mouse IgG1/PE negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD27 antibody. Conjugation: PE
Production: Protein A purified antibody from tissue culture supernatant was conjugated to R-Phycoerythrin through a sulfo-ester linkage. Unconjugated antibody was removed using size exclusion chromatography. Antibody conjugate is at 0.25 mg/ml with an OD565/OD280 ratio of 2.9.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 15% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: T cell line (from patient with CLL). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD27 are readily searchable from our website. Different antibodies against the same target such as CD27 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.