anti-CD27 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD27; anti-CD27 antibody;
The CD27 cd27 (Catalog #MBS834589) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD27 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: <= 0.5 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD27 cd27 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD27 cd27 product has the following accession number(s) (GI #4507587) (NCBI Accession #NP_001233.1) (Uniprot Accession #P26842). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD27 antibody (FITC) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of C57Bl/6 splenocytes with CD45R antibody (eFluor 450) and 0.25 ug of Armenian Hamster IgG Isotype Control (FITC) (left) or 0.25 ug of CD27 antibody (FITC) (right). Total viable cells were used for analysis.)
Armenian Hamster monoclonal CD27 antibody (FITC).
Biological Significance: The LG.7F9 monoclonal antibody reacts with mouse CD27, a lymphocyte-specific member of the TNFR superfamily. CD27 is expressed by virtually all mature T cells and by a subpopulation of B cells, mainly memory B cells. In mouse, CD27 has been found on nearly all thymocytes excluding a population of CD46-CD8- precursors. CD27 binds to CD70 and, through this interaction, plays an important role in T cell-B cell interaction. It has been reported that triggering CD27 plays an important role in the maturation of CD4 and CD8 effector cells. LG.7F9 cross-reacts with human and rat CD27. Tag/Conjugate: FITC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD27 are readily searchable from our website. Different antibodies against the same target such as CD27 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Heart, Intestine, Kidney, Lung, Lymph, Lymph Node, Spleen, Thymus, Tonsil tissues are correlated with this protein. The following patways have been known to be associated with this gene. CD27 also interacts with the following gene(s): CCR3, CD40LG, CD5, CD70, CXCR3, IL10, SIVA1, TRAF2, TRAF3. Carcinoma, Inflammation, Kidney Diseases, Liver Diseases, Lymphoma, Memory Disorders, Necrosis, Neoplasms, Experimental, Nervous System Diseases, Skin Diseases are some of the diseases may be linked to CD27 antibody (FITC).