anti-CD273 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD273; anti-CD273 antibody;
The CD273 n/a (Catalog #MBS666527) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD273(B7-DC1)-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD273 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Blocking. Researchers should empirically determine the suitability of the CD273 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD273(B7-DC1)-Biotin with the following immunoassay(s):
Testing Data
Information: CD273 (B7-DC, PD-L2, Programmed Death Ligand 2) is a 25 kd member of the B7 family and is expressed mainly on a sub set of Dendritic cells. When engaged it has a stimulatory effect on the expressing DC, and delivers a down regulatory signal to cells expressing its counter receptor, CD279(PD-1). The interaction of CD273(B7-DC) and CD274(B7-H1) with their receptor, CD279(PD-1) is an important component of immune regulation. Antibody ANC8D12 binds to recombinant CD273 and cell surface CD273 expressed on monocyte derived Dendritic cells (moDC), and blocks binding of recombinant CD279 to CD273 in EIA and FACS.
Performance: Human monocyte derived Dendritic cells were prepared in 8 day culture. Five x 105 cells per tube were washed and incubated 45 minutes on ice with 80 ul of anti-human CD273/Biotin at concentration of 10 ug/ml. They were then washed twice and incubated with Streptavidin/R-PE, after which they were washed twice, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.39 log10 fluorescent units when compared to isotype control Mouse IgG2a/Biotin. Binding was blocked when reagent was pre incubated with a 10-fold excess of recombinant CD273-muIg. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Recombinant� human CD273(B7-DC). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD273 are readily searchable from our website. Different antibodies against the same target such as CD273 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.