anti-CD278 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD278; anti-CD278 antibody;
The CD278 n/a (Catalog #MBS666514) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD278(ICOS)-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD278 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA). Researchers should empirically determine the suitability of the CD278 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD278(ICOS)-Biotin with the following immunoassay(s):
Testing Data
Information: The inducible costimulator CD278 (ICOS, T cell activation molecule H4) is similar to human CD28 (24% homology), and plays an analogous role in the T cell activation process. Secondary signaling through CD28 or ICOS results in discrete cytokine secretion profiles by the activated T cells.1 Signaling by either molecule is effectively down regulated by CD152 (CTLA-4) engagement.2 Human CD275 (GL50, B7-H2) is a member of the B7 family sharing ~20% homology with CD80 (B7-1) and CD86 (B7-2), and has been shown to be a ligand for ICOS.3 Two RNA splice variants exist for this molecule, differing only in the cytoplasmic domain. 4 Antibody ANC6C6 binds to recombinant ICOS in EIA, and to the surface of stimulated PBL and HPB-MLT tumor cells in Flow cytometry. Additionally, it blocks binding of recombinant CD275-muIg to HPB-MLT cells.
Performance: Ficoll prepared human peripheral blood mononuclear cells were stimulated 5 days in culture with 5 ug/ml PHA after which they were harvested and washed in FACS buffer. Five x 105 cells per tube were pre incubated 5 minutes with 20 ul of 300 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ml of anti-CD278/Biotin at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed three times, fixed and analyzed by FACS using a lymphoid gate. Cells stained positive with a mean shift of 1.53 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD278 antibody. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human HPB-MLT cells, recombinant human CD278(ICOS)-muIg. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD278 are readily searchable from our website. Different antibodies against the same target such as CD278 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.