|The CD28 cd28 (Catalog #MBS211255) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD28 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN).
Flow Cytometry: Use10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD28 cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD28 cd28 product has the following accession number(s) (GI #161484597) (NCBI Accession #NP_031668.3) (Uniprot Accession #P31041). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Mouse anti Mouse CD28 antibody, clone E18 recognizes murine CD28, a co-stimulatory cell surface molecule expressed by T cell subsets. Clone E18 has been shown to be able to stimulate murine T lymphocytes in combination with anti-mouse CD3.
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Alternate immunisation with A20 cells expressing mCD28 and recombinant mCD28Ig.
Fusion Partners: Spleen cells from immunised CD28-/-knockout mice were fused with cells of the X63Ag8.653 myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD28 are readily searchable from our website. Different antibodies against the same target such as CD28 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CD28 also interacts with the following gene(s): Cd80, Cd86, Grap2, Grb2, Lck, Pik3r3. The following patways have been known to be associated with this gene. Bone, Bone Marrow, Embryonic Tissue, Joint, Lung, Mammary Gland, Skin, Spleen, Thymus tissues are correlated with this protein. Arthritis, Rheumatoid, Asthma, Autoimmune Diseases, Carcinoma, HIV Infections, Inflammation, Liver Diseases, Lung Diseases, Lymphoma, Necrosis are some of the diseases may be linked to MOUSE ANTI MOUSE CD28:Low Endotoxin.