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anti-CD28 antibody product blog

Posted on 2015-09-21 23:32:20 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD28; anti-CD28 antibody;
The CD28 cd28 (Catalog #MBS213120) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD28 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells or 100ul whole blood.
Flow Cytometry: Minimum Dilution: 1/25; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD28 cd28 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD28 cd28 product has the following accession number(s) (GI #340545508) (NCBI Accession #NP_001230006.1) (Uniprot Accession #P10747). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI HUMAN CD28 with the following immunoassay(s):
Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Biotin (MBS210509))
Testing Data CD28.

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28:RPE (MBS213865))
Testing Data CD28.

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28: Alexa Fluor 647 (MBS2141859A647))
Testing Data CD28.

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD28)
Testing Data CD28.

Rat anti Human CD28 antibody, clone YTH913.12 recognizes human CD28, a ~44kDa single pass type 1 trans-membrane protein expressed as a homodimer on a major subset of human T-cells (Thompson et al. 1989), responsible for activation of these cells via interaction with the TCR. CD28 is involved in the tuning of the T-cell for activation via TCR, lowering the threshold for activation from around 8000 triggered TCRs to approximately 1500 (Viola et al.1996). CD28 along with CD152, also known as CTLA-4 acts as a co-receptor for the co-stimulatory molecules CD80 and CD86 (Azuma et al. 1993). CD28 offers a positive stimulatory role on ligation of CD80 and CD86 while CTLA-4 offers a negative feedback signal preventing CD28 mediated T-cell activation of CD86 (Krummel et al. 1995) Rat anti human CD28, clone YTH913.12 has been reported to recognize an epitope of CD28 expressed by NK cells, which is not recognized by other anti human CD28 clones such as 9.3 and CD28.2 (Galea-Lauri et al 1999.) Other reports however have failed to demonstrate CD28 staining on peripheral blood derived KK cells using clone YTH913.12 (Wilson et al. 1999).

Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Buffer Solution: Phosphate buffered saline
Target Species: Human. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD28 are readily searchable from our website. Different antibodies against the same target such as CD28 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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