anti-CD29 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD29; anti-CD29 antibody;
The CD29 itgb1 (Catalog #MBS211228) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD29 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Functional Assays (FN), Immunoprecipitation (IP).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100. Researchers should empirically determine the suitability of the CD29 itgb1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD29 itgb1 product has the following accession number(s) (GI #45504394) (NCBI Accession #NP_034708.1) (Uniprot Accession #P09055). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the HAMSTER ANTI MOUSE CD29:Low Endotoxin with the following immunoassay(s):
Testing Data (Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29 (MBS212672))
Testing Data (Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29:FITC (MBS210933))
Testing Data (Staining of mouse spleen cells with Hamster anti Mouse CD29: Alexa Fluor 488 (MBS212672A488))
Testing Data (Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29 (MBS212673))
Testing Data (Staining of mouse spleen cells with Hamster anti Mouse CD29: Alexa Fluor 647 (MBS212672A647))
Testing Data (Staining of mouse peripheral blood lymphocytes with Hamster anti Mouse CD29: Low Endotoxin)
Hamster anti Mouse CD29 antibody, clone HM beta 1-1 recognizes the murine integrin beta 1 subunit (CD29), a 110kDa cell surface glycoprotein that is widely expressed by a variety of cells including all leucocytes. CD29 forms non-covalent bonds with the integrin alpha subunits, including CD51 and CD49a-f, to form heterodimers. The ligands for these heterodimers include collagen, fibronectin, laminin and vascular adhesion molecule-1. In the immune system beta 1 integrins play an important role in cell adhesion, migration, activation and differentiation. Hamster anti Mouse CD29 antibody, clone HM beta 1-1 is reported to inhibit beta 1 integrin mediated adhesion (Noto et al. 1995).
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Purified mouse VLA-4 antigen
Fusion Partners: Spleen cells from immunised Armenian hamsters were fused with cells of the P3U1 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD29 are readily searchable from our website. Different antibodies against the same target such as CD29 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.