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anti-CD3 antibody product blog

Posted on 2015-11-22 21:13:26 by mybiosource_staff
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Tags: Antibody; CD3; Monoclonal Antibody; anti-CD3 antibody;
The CD3 cd3e (Catalog #MBS219799) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD3 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS)*, Immunofluorescence (IF), Immunohistology Paraffin*.
Immunohistology - Frozen: Maximum Dilution: 1/100;
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/100; Application Note: Membrane permeabilization is required for this application. MyBioSource recommends the use of Leucoperm for this purpose.
Immunohistology - Paraffin: Maximum Dilution: 1/100; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Tris/EDTA buffer pH 9.0 is recommended for this purpose. Researchers should empirically determine the suitability of the CD3 cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD3 cd3e product has the following accession number(s) (GI #4502671) (NCBI Accession #NP_000724.1) (Uniprot Accession #P07766). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI HUMAN CD3 with the following immunoassay(s):
Testing Data (Western blot analysis of Jurkat human acute T-cell leukemia whole cell lysate probed with Mouse anti Human CD3 antibody (MBS215051) followed by HRP conjugated Goat anti Mouse IgG, visualized by chemiluminescence)
Testing Data CD3.

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD3 (MBS215051) following permeabilisation with Leucoperm)
Testing Data CD3.

Testing Data (ELISA analysis of human CD3 expression using Rat anti Human CD3ε, clone CD3-12 as a capture reagent and biotinylated Mouse anti Human CD3, clone UCHT1 (MBS219235G) as a detection reagent with recombinant human CD3 as antigen to produce the standard curve. Detection is by HRP conjugated streptavidin and substrate. Microtitre plate is read at O.D. 450 nm on the iMark Microplate Absorbance Reader. A serum (green) sample is included undiluted)
Testing Data CD3.

Testing Data (Formalin fixed, paraffin embedded human tonsil stained with Rat anti Human CD3 (MBS215051) following heat mediated antigen retrieval in EDTA buffer pH9.0)
Testing Data CD3.

Testing Data (Staining of human peripheral blood lymphocytes with Rat anti Human CD3 (MBS215051). Permeabilised with Leucoperm)
Testing Data CD3.

Rat anti Human CD3, clone CD3-12 raised against a peptide representing an invariant cytoplasmic sequence within the CD3ε chain recognizes human CD3ε. CD3 is a multimeric protein complex composed of four distinct polypeptide chains (ε, gamma, delta, ζ) that assemble and function as three pairs of dimers (ε gamma, εdelta, ζζ). The CD3 complex serves as a T cell co-receptor that associates non-covalently with the T cell receptor (TCR) (Malissen 2008; Guy and Vignali 2009; Smith-Garvin et al. 2009). CD3 is a defining feature of cells belonging to the T cell lineage and can therefore be used as T cell marker. As Rat anti Human CD3, clone CD3-12 has been specifically raised against an epitope within the epsilon peptide chain, highly conserved among species clone CD3-12 has a very broad species crossreactivity for the CD3 marker. (Jones et al. 1993; Kothlow et al. 2005).

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