anti-CD3 antibody product blog
Tags: Antibody; CD3; Monoclonal Antibody; anti-CD3 antibody;
The CD3 cd3g (Catalog #MBS666729) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD3-Biotin reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD3 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), PS. Researchers should empirically determine the suitability of the CD3 cd3g for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD3 cd3g product has the following accession number(s) (GI #1914807) (NCBI Accession #CAA72995.1) (Uniprot Accession #P79951). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD3-Biotin with the following immunoassay(s):
Testing Data
Information: The human CD3/T cell receptor (TcR) complex is made up of at least five CD3 proteins (gamma, delta, epsilon, h, z) in association with either alpha/beta or gamma/delta proteins of the TcR. The TcR recognizes antigens in association with MHC molecules after which protein chains of the CD3 complex mediate activation signals triggered by TcR antigen binding. CD3 is expressed on greater than 95% of circulating human peripheral T cells. Antibody UCHT1 recognizes the 20 kd episilon chain of the CD3 molecule complex. Antibody UCHT1 will activate T cells expressing CD3epsilon.
Performance: Five x 105 cultured HPB-MLT cells were washed and incubated 45 minutes on ice with 80 ul of anti-CD3/Biotin at a concentration of 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Streptavidin/R-Phycoerythrin, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 2.18 log10 fluorescent units when compared to a Mouse IgG1/Biotin negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with anti-CD3 antibody. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein A purified to >95% mouse immunoglobulin by SDS-PAGE (<1% bovine immunoglobulin), and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human thymocytes/Sezary T cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD3 are readily searchable from our website. Different antibodies against the same target such as CD3 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Anemia, Carcinoma, Hepatocellular, Inflammation, Liver Diseases, Liver Neoplasms, Neoplasms, Experimental are some of the diseases may be linked to anti-human CD3-Biotin.