anti-CD31 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD31; anti-CD31 antibody;
The CD31 n/a (Catalog #MBS224540) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD31 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS), Immunoprecipitation (IP).Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/25. Researchers should empirically determine the suitability of the CD31 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI SHEEP CD31 with the following immunoassay(s):
Testing Data #1 (Staining of sheep peripheral blood platelets with Mouse anti Ovine CD31 (MBS224540))
Mouse anti Sheep CD31 antibody, clone CO.3E1D4 recognizes ovine CD31, also known as PECAM-1. Ovine CD31 is predominantly expressed by peripheral blood platelets and a small percentage of lymphocytes. CD31 is also highly expressed by ovine endothelial cells. Mouse anti Sheep CD31 antibody, clone CO.3E1D4 is reported to inhibit homotypic leucocyte aggregation induced by anti CD43 antibodies (Pintado et al. 1995).
Perservative Stabilisers: 0.09% Sodium Azide (NaN3)
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant. Immunogen: Ovine leucocytes
Fusion Partners: Spleen cells from immunised BALB/c mice were fused with cells of the SP2-0/Ag14 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Sheep. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD31 are readily searchable from our website. Different antibodies against the same target such as CD31 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.