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anti-CD31 antibody product blog

Posted on 2018-03-19 07:36:39 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD31; anti-CD31 antibody;
The CD31 pecam1 (Catalog #MBS669024) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Monoclonal Antibody to CD31 (Clone: PECAM-1) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD31 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC), Flow Cytometry (FC/FACS).
IHC analysis: 1: 25-1: 100
FC/FACS: 0.5ug/10^6 cells. Researchers should empirically determine the suitability of the CD31 pecam1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD31 pecam1 product has the following accession number(s) (GI #313760624) (NCBI Accession #NP_000433.4) (Uniprot Accession #P16284). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Monoclonal Antibody to CD31 (Clone: PECAM-1) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Fig.1: FLOW Cytometry analysis of CD31 in human PBMC using 0.5ug antibody per 10^6 cells. Green represents isotope control. Red represents anti-CD31 antibody (Clone: PECAM-1). Goat anti-mouse IgG conjugated with PE was used as secondary antibody.)
Flow Cytometry (FC/FACS) CD31.

Immunohistochemistry (IHC) (Fig: 2 Immunohistochemical analysis of CD31 in human lung squamous cell carcinoma (left) and bladder transitional cell carcinoma (right) using CD31 antibody (Clone: PECAM-1) at 1: 200 dilution.)
Immunohistochemistry (IHC) CD31.

CD31 (PECAM-1) is a transmembrane glycoprotein member of the immunoglobulin supergene family of adhesion molecules, and plays key roles in leukocyte migration, angiogenesis, and integrin activation. CD31 is expressed on endothelial and hematopoeitic (platelets, monocytes, macrophages, granulocytes, T and B lymphocytes, dendritic, bone marrow stem and adult) cells.

Antigen: CD31
Immunogen Information: Human spleen membranes from a patient with hairy cell leukemia was used as immunogen to generate the CD31 (PECAM-1) antibody.
Conjugation: Unconjugated. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD31 are readily searchable from our website. Different antibodies against the same target such as CD31 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. The following patways have been known to be associated with this gene. Breast Neoplasms, Heart Diseases, Inflammation, Liver Diseases, Lung Diseases, Lung Neoplasms, Necrosis, Neoplasm Metastasis, Neoplasms, Neoplasms, Experimental are some of the diseases may be linked to Monoclonal Antibody to CD31 (Clone: PECAM-1).
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