anti-CD34 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD34; anti-CD34 antibody;
The CD34 cd34 (Catalog #MBS224400) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD34 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 1 x 106 cells in 100ul.
Flow Cytometry: Maximum Dilution: Neat. Researchers should empirically determine the suitability of the CD34 cd34 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD34 cd34 product has the following accession number(s) (GI #68342038) (NCBI Accession #NP_001020280.1) (Uniprot Accession #P28906). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD34:RPE with the following immunoassay(s):
Testing Data #1 (Paraffin processed placenta stained with Mouse anti Human CD34 Class II (MBS224445))
Mouse anti Human CD34 antibody, clone QBEND/10 recognizes the human CD34 antigen, also known as Hematopoietic progenitor cell antigen CD34. Human CD34 is 385 amino acid polypeptide containing a 31 residue signal peptide, cleaved to yield the ~110kDa mature form of CD34, a sialomucin single pass transmembrane glycoprotein. CD34 is expressed by stem cells (Kaufman et al. 2001) and small vessel endothlium (Ramani et al. 1990) Human CD34 exists as two isoforms, the full length form described here and a truncated isoform lacking the carboxy-terminal of the intracellular domain and containing some alternative sequence in the remaining intracellular region. Antibody binding epitopes on human CD34 have been classified according to their resistance to enzymatic degradation and grouped together using this and competitive binding assays (Lanza et al. 1999). Clone QBEND/10 has been classified as binding to the class II epitope, resistant to neuraminidase treatment but sensitive to both glycoprotease and chymopapain digestion. Clone QBEND/10 binds to a different eoptope to clone 581 which binds to the class III epitope resistant to all three enzymzatic treatments (Nishio et al. 1996 In Leukocyte tTyping VI). Clone QBEND 10 is expected to bind to both isoforms of human CD34 as it\'s binding epitope has been mapped to the extracellular domain between amino acids 43 and 49 by peptide microarray analysis (Jones et al. 1996, in Leukocyte Typing VI). Mouse anti Human CD34 antibofy, clone QBEND/10 has been succesfully exploited foir the detection of CD34 in brain capillaries of Alzheimer\'s patients (Kalaria et al. 1992) and in acute lymphoblastic leukemia cells (Sutherland et al. 1992) by Western blotting.
Reconstitution: Reconstitute with 1 ml distilled water
Perservative Stabilisers: 0.09% Sodium Azide
1% Bovine Serum Albumin
Preparation: Purified IgG prepared by affinity chromatography on Protein A from tissue culture supernatant.