|The CD35 n/a (Catalog #MBS666173) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD35-Purified Preservative Free reacts with Human, Monkey and may cross-react with other species as described in the data sheet. MyBioSource\'s CD35 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB). Researchers should empirically determine the suitability of the CD35 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD35-Purified Preservative Free with the following immunoassay(s):
Information: Human CD35 binds complement components C3b, C4b, and iC3b and is expressed on erythrocytes, B cells, monocytes, granulocytes and folicular dendritic cells and on subsets of thymocytes. CD35 on erythrocytes mediates clearance of immunue complexes. Antibody E11 recognizes a complement receptor 1 (CD35) molecule of about 220 kd.
Performance: Five x 105 cultured U-937 human tumor cells were washed and pre incubated 5 minutes with 20 ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD35 antibody at a concentration of 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.70 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2u sterile filtered and vialed under aseptic conditions.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen: Human monocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD35 are readily searchable from our website. Different antibodies against the same target such as CD35 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.