Antibody Protein ELISA Kit from MyBioSource header Telephone 1.858.633.0165          
Fax 1.858.633.0166    Email 
menu1 Home Products Ordering Company Help menu7
   MyBioSource  rightarrow Monoclonal Antibody Blog  rightarrow anti-CD357 antibody  rightarrow LOG IN  rightarrow MY ACCOUNT  rightarrow CART CONTENTS  rightarrow CHECKOUT 
horiz bar
MyBioSource Blogs moreseparator
 • anti-CNTN5 antibody blog
 • anti-CD143 antibody blog
 • anti-CD24/HSA antibody blog
 • anti-ESM1 antibody blog
 • anti-NUP133 antibody blog
 • anti-Influenza B antibody blog
 • anti-F4/80 antibody blog
 • anti-Hepatitis C virus antibody blog
 • anti-Gucy2c antibody blog
 • anti-ALOX15 antibody blog
 • anti-Kcnb1 antibody blog
 • anti-HSP90AA1 antibody blog
 • anti-CRMP2 antibody blog
 • anti-GSNOR, ADH5 antibody blog
 • anti-S100P antibody blog
 • CD4 antibody blog
 • anti-KCNA3 antibody blog
 • anti-NMDAR1 antibody blog
 • CD1A antibody blog
 • anti-PEX6 antibody blog
Blog Categories separator
 • Assay Kit Blogs
 • Biochemical Blogs
 • cDNA Clone Blogs
 • Monoclonal Antibody Blogs
 • Polyclonal Antibody Blogs
 • Rec/Purified Protein Blogs
 • Popular Product Blogs
Our Best Sellers moreseparator
 • Rabies Virus Antibody
 • DNA Polymerase zeta (REV3L) Antibody
 • lysophospholipase-like 1 (LYPLAL1) ELISA Kit
 • Galectin-3-binding protein (Lgals3bp) ELISA Kit
 • Bone morphogenetic protein 8B (BMP8B) Recombinant Protein
 • gasdermin D (GSDMD) ELISA Kit
 • Myosin Heavy Chain 8, Skeletal Muscle, Perinatal (MYH8) ELISA Kit
 • intercellular adhesion molecule 1, ICAM-1-CD54 ELISA Kit
 • Corticotropin Releasing Hormone (CRH) ELISA Kit
 • Mast Cell Chymase (CMA1) Antibody
 • Synuclein-pan Antibody
 • Asymmetric dimethylarginine (ADMA) ELISA Kit
 • Epinephrine-Adrenaline ELISA Kit
 downarrow more ...

anti-CD357 antibody product blog

Posted on 2015-12-01 17:28:06 by mybiosource_staff
Share it on ...     
Tags: Antibody; Monoclonal Antibody; CD357; anti-CD357 antibody;
The CD357 n/a (Catalog #MBS666414) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD357(GITR)-FITC reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD357 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA).
1:50 (or use 1.6 ul of concentrated reagent per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD357 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD357(GITR)-FITC with the following immunoassay(s):
Testing Data
Testing Data CD357.

Information: Glucocorticoid induced Tumor Necrosis Factor receptor family related gene (CD357, GITR, AITR) is a type 1 228 amino acid transmembrane protein that has been designated TNFRSF18. GITR is expressed mainly by CD4+ CD25+ T lymphocytes. Its engagement by antibodies or its ligand (GITRL) induces a costimulatory signal which can break T cell suppression (2). GITR has been implicated as a regulatory mechanism in anti-parasitic immune resposes (4), and is involved with Macrophages activation in atherosclerotic plaque formation (3). Clone ANC7D6-1E7 binds to human CD357 in EIA and on stimulated cell surface in FACS. Pre incubation of the receptor with an excess of this mAb will block binding of recombinant GITR Ligand.

Performance: Ficoll prepared human peripheral blood mononuclear cells (PBMC) were stimulated 5 days in culture with PHA. Five x 105 cells per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-GITR/FITC at a 1:50 dilution factor (10ug/ml). Cells were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.03 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated with a 10-fold excess of unlabeled anti-GITR mAb. Conjugation: FITC
Production: Antibody from (low FBS containing) tissue culture supernatant was purified by size exclusion chromatographand reacted with FITC. Unconjugated FITC was removed from conjugate using a desalting column. Antibody conjugate is at 0.5 mg/ml with a Fluorescein/IgM molar ratio of 35.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Recombinant human GITR, PHA stimulated human PBL. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD357 are readily searchable from our website. Different antibodies against the same target such as CD357 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
horiz bar
 SSL   Follow us on Facebook Follow us onTwitter Follow us on Google Plus Connect us on LinkedIn Subscribe to our RSS Feed for latest products and special promotions