|The CD36 cd36 (Catalog #MBS666306) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD36-FITC reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD36 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunoprecipitation (IP).
1:50 (or use 1.6 ul of concentrated stock per 5 x 105-cell test). Researchers should empirically determine the suitability of the CD36 cd36 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD36 cd36 product has the following accession number(s) (GI #12620274) (NCBI Accession #AAG60639.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD36-FITC with the following immunoassay(s):
Information: Human CD36 is a major platelet glycoprotein also found on monocytes, macrophages and some endothelial cells. CD36 may play a role in cell adhesion as a receptor for thrombospondin. CD36 is a receptor for adhesion of malaria infected erythrocytes. CD36 is also proported to have a role in phagocytosis. Antibody SMO recognizes the CD36 molecule of 88 kd.
Performance: Five x 105 ficoll prepared human peripheral white blood cells were washed and pre incubated 5 minutes with 20ul of 250ug/ml human IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD36/FITC at a 1:50 dilution (10 ug/ml). Cells were then washed three times, fixed and analyzed by FACS. A net 5.5% sub population of the cells stained positive with a mean shift of 1.8 log10 fluorescent units when compared to a Mouse IgM/FITC negative control at a similar concentration. Binding was blocked when cells were pre incubated 10 minutes with 20 ul of 0.5 mg/ml anti-CD36 antibody. Conjugation: FITC
Production: Antibody from (low FBS containing) tissue culture supernatant was size exclusion purified to 95% Immunoglobulin by SDS-PAGE, and reacted with fluorescein. Unconjugated fluorescein was separated from antibody conjugate by desalting column. The conjugate is at 0.5 mg/ml.
Buffer: 50 mM Sodium Phosphate pH 7.5, 500 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Human tonsil cells /PBM. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD36 are readily searchable from our website. Different antibodies against the same target such as CD36 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.