|The CD3E cd3e (Catalog #MBS200286) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD3E antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD3E can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF).
Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the CD3E cd3e for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD3E cd3e product has the following accession number(s) (GI #4502671) (NCBI Accession #NP_000724) (Uniprot Accession #P07766). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD3E antibody with the following immunoassay(s):
Western Blot (WB) (Fig. 1: The extract of Jurkat (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system. Fig. 2: The Human Recombinant protein CD3E (200, 100, 50ng) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD3E antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of CD3E in Jurkat cells line, stained with DAPI (Blue) for nucleus staining and monoclonal anti-human CD3E antibody (1:100) with goat anti-mouse IgG-Alexa fluor 488 conjugate (Green).)
The T cell antigen receptor (TCR) recognizes foreign antigens and translates such recognition events into intracellular signals that elicit a change in the cell from a dormant to an activated state. Much of this signaling process can be attributed to a multi subunit complex of proteins that associates directly with the TCR. This complex has been designated CD3 (cluster of differentiation 3). It is composed of five invariant polypeptide chains that associate to form three dimers. The epsilon polypeptide plays an essential role in T-cell development. Defects in this gene cause immunodeficiency. This gene has also been linked to a susceptibility to type I diabetes in women.
Antigen Species: Human
Immunogen: Recombinant human CD3E (23-126aa) purified from E. coli. Clone Note: Anti-human CD3E mAb, clone AT3H3, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD3E protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD3E are readily searchable from our website. Different antibodies against the same target such as CD3E may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.