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anti-CD4 antibody product blog

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Posted on 2016-01-11 12:40:23 by mybiosource_staff
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Tags: Antibody; CD4; Monoclonal Antibody; anti-CD4 antibody;
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The CD4 cd4 (Catalog #MBS219363) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD4 can be used in a range of immunoassay formats including, but not limited to, Flow cytometry (FC/FACS).
Flow Cytometry: Use 10ul of the suggested working dilution to label 1x106 cells in 100ul. The Fc region of monoclonal antibodies may bind non-specifically to cells expressing low affinity Fc receptors. This may be reduced by using SeroBlock FcR.
Flow Cytometry: Minimum Dilution: Neat. Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD4 cd4 product has the following accession number(s) (GI #7304953) (NCBI Accession #NP_038516.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD4:RPE with the following immunoassay(s):
Testing Data (Staining of mouse spleen with Rat anti Mouse CD4 (MBS219566))
Testing Data CD4.

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Medium power)
Testing Data CD4.

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). High power)
Testing Data CD4.

Testing Data (Immunoperoxidase staining of a mouse lymph node cryosection with Rat anti Mouse CD4 antibody, clone GK1.5 (MBS219566) followed by horseradish peroxidase conjugated Goat anti Rat IgG (MBS235195). Low power)
Testing Data CD4.

Testing Data (Published customer image: Intrafollicular location of MZB cells in B6.TC spleens. A. Representative spleen sections from 3 mo old B6.TC (left) and B6 (right) mice stained with Moma-1-FITC, CD1d-PE and B220-PB. The B220+ CD1d+ MZB cells show as bright pink while the other B cells show as blue. The ring of Moma-1+ metallophillic macrophages delineates the MZ inner edge. B. Percentage of CD1d+ B220+ B cells relative to total B220+ B cells outside (MZ) and inside (FO) the Moma-1+ ring in 3 mo and 10 mo old B6 and B6.TC mice. The data show means and standard errors of the mean (SEM) calculated of 4 MZ and FO areas for each mouse. ***: p < 0.001 for t tests. C. Representative spleen sections from 10 mo old B6.TC (left) and B6 (right) mice stained with CD4-FITC, CD1d-PE and B220-PB. Boxed areas show multiple contacts between green B6.TC CD4 T cells and pink MZB cells, but not in the B6 spleen. Original magnification: 200X.From: Zhou et al. BMC Immunology 2011 12:7.)
Testing Data CD4.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4 Alexa Fluor ?647 (MBS219566A647))
Testing Data CD4.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:FITC (MBS219462))
Testing Data CD4.

Testing Data (Staining of mouse peripheral blood lymphocytes with Rat anti Mouse CD4:Low Endotoxin (MBS219398))
Testing Data CD4.

Testing Data (Staining of mouse spleen with Rat anti Mouse CD4:Alexa Fluor ?488 (MBS219566A488))
Testing Data CD4.

Testing Data (Staining of mouse spleen with Rat anti Mouse CD4:RPE (MBS219761))
Testing Data CD4.

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