|The CD4 cd4 (Catalog #MBS666215) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The anti-mouse CD4-Biotin reacts with Mouse and may cross-react with other species as described in the data sheet. MyBioSource\'s CD4 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), ELISA (EIA), Immunohistochemistry (IHC), Immunoprecipitation (IP). Researchers should empirically determine the suitability of the CD4 cd4 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD4 cd4 product has the following accession number(s) (GI #3947700) (NCBI Accession #CAA60883.1) (Uniprot Accession #Q13969). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-mouse CD4-Biotin with the following immunoassay(s):
Information: Antibody GK1.5 binds to an extracellular epitope of the 55kd CD4 molecule present on a murine T cell subset (1,2). CD4 binds to MHC Class II expressed by antigen presenting cells, facilitating T Cell Receptor signaling. This clone has proven effective as a T cell depletion reagent(3).
Performance: Reagent was tested for binding to ACK lysed murine splenocytes in FACS. Five x 105 splenocytes per tube were washed and pre incubated 5 minutes with 20 ul of 250 ug/ml Mouse IgG (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-mouse CD4/Biotin at concentration of 1 ug/ml. They were then washed twice and incubated with 2 degree reagent Streptavidin/R-PE after which they were washed twice, fixed and analyzed by FACS. A 24% sub population of the cells stained positive with a mean shift of 1.78 log10 fluorescent units when compared to an Isotype control Mouse IgG1/Biotin. Binding was blocked when cells were pre incubated with 20ul of 0.5mg/ml unlabeled anti-CD4 antibody. Conjugation: Biotin
Production: Antibody from (low FBS containing) tissue culture supernatant was Protein G purified and reacted with NHS-Biotin. Unconjugated Biotin was removed from conjugate using a desalting column.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 5% Glycerol, 0.2% BSA, 0.04% NaN3 (as a preservative).
Immunogen: Mouse CTL-V4 membrane prep. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD4 are readily searchable from our website. Different antibodies against the same target such as CD4 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.