anti-CD40 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD40; anti-CD40 antibody;
The CD40 cd40 (Catalog #MBS212039) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD40 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, ELISA (EIA), Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/10; Maximum Dilution: 1/50. Researchers should empirically determine the suitability of the CD40 cd40 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD40 cd40 product has the following accession number(s) (GI #70608202) (NCBI Accession #NP_035741.2) (Uniprot Accession #P27512). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the RAT ANTI MOUSE CD40 with the following immunoassay(s):
Testing Data (Staining of mouse splenocytes with Rat anti Mouse CD40: Low Endotoxin (MBS211191))
Testing Data (Staining of mouse splenocytes with Rat anti Mouse CD40: Alexa Fluor 647 (MBS212038A647))
Testing Data (Staining of mouse splenocytes cells with Rat anti Mouse CD40 (MBS212038))
Testing Data (Staining of mouse splenocytes with Rat anti Mouse CD40:RPE (MBS213555))
Testing Data (Staining of mouse splenocytes with Rat anti Mouse CD40:FITC (MBS210623))
Testing Data (Staining of mouse splenocytes with Rat anti Mouse CD40: RPE (MBS213556))
Rat anti Mouse CD40 antibody, clone 3/23 recognizes the murine CD40 cell surface glycoprotein. It does not react with normal mouse Ig or with human IgG1 and will stain most mature mouse B cells. It does not cross react with mouse T cells. The specificity of Rat anti Mouse CD40 antibody, clone 3/23 was demonstrated by ELISA and flow cytometry using BHK cells transfected with mouse CD40. Rat anti Mouse CD40 antibody, clone 3/23 is a powerful activator of normal B cells especially in the presence of IL-4.
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant. Immunogen: Extracellular Domain of Mouse CD40 and the Fc portion of Human IgG1
Fusion Partners: Spleen cells from immunised LOU/c rats were fused with cells of the Ag8 mouse myeloma cell line.
Buffer Solution: Phosphate buffered saline
Target Species: Mouse. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD40 are readily searchable from our website. Different antibodies against the same target such as CD40 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.