|The CD45R/B220 n/a (Catalog #MBS2544748) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. The Anti-Mouse/Human CD45R/B220 monoclonal antibody (PerCP/Cy5.5 conjugated) reacts with Mouse, Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD45R/B220 can be used in a range of immunoassay formats including, but not limited to, Flow Cyometry (FC/FACS).
Each lot of this antibody is quality control tested by immunofluorescent staining with flow cytometric analysis (The amount of the reagent is suggested to be used <= 0.25 ug /10^6 cells in 100 ul). Since applications vary, the appropriate dilutions must be determined for individual use. Researchers should empirically determine the suitability of the CD45R/B220 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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CD45R, also known as B220, is an isoform of CD45. It is a member of the protein tyrosine phosphatase (PTP) family with a molecular weight of approximately 180-240 kD. CD45R is expressed on B cells (at all developmental stages from pro-B cells through mature B cells), activated B cells, and subsets of T and NK cells. CD45R (B220) is also expressed on a subset of abnormal T cells involved in the pathogenesis of systemic autoimmunity in MRL-Faslpr and MRL-Fasgld mice. It plays a critical role in TCR and BCR signaling. The primary ligands for CD45 are galectin-1, CD2, CD3, and CD4. CD45R is commonly used as a pan-B cell marker; however, CD19 may be more appropriate for B cell specificity.
Buffer: PBS pH 7.2, 0.09% NaN3, 0.2% BSA. Conjugation: PerCP/Cy5.5. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD45R/B220 are readily searchable from our website. Different antibodies against the same target such as CD45R/B220 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.