anti-CD45RO antibody product blog
Tags: Antibody; Monoclonal Antibody; CD45RO; anti-CD45RO antibody;
The CD45RO n/a (Catalog #MBS8505713) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse anti-Human CD45RO, FITC Conjugated mAb reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD45RO can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Immunofluorescence (IF). Researchers should empirically determine the suitability of the CD45RO n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
4AUCHL1 reacts with an isoform of CD45, a 180 kDa molecule clustered as CD45RO. CD45RO is expressed on memory/activated T cells and cortical thymocytes, in comparison with CD45RA, which is expressed on naive/resting T cells and on medullart thymocytes. CD45RO and CD45RA molecules are important to discriminate naive and memory T cells in the study of the immune system.
Experimental Methods: 1.Take 100mul peripheral blood anticoagulated by EDTA and add to the bottom of 5ml tube;
2.Add 10mul labeled antibody to the bottom of flow tube mixing with the whole blood, incubate for 20 minutes at room temperature away from light;
3.Add 2 ml1�RBC lysis buffer, incubate for 10 minutes away from light after mixing, dissolve red blood cells (recommended: RBC lysing Solution 10�);
4.Sample tube is set to 1000 rpm centrifugation for 5 minutes, discard the supernatant;
5.Add 2 ml PBS wash buffer to resuspend the cells, then1000 rpm centrifugation for 5 minutes, discard the supernatant;
6.Add 0.5 ml PBS wash buffer to resuspend the cells and detect by flow cytometry (sample should be determined on the day on the machine and can also be added fixation overnight at 4 degree C then measured).
[PBS wash buffer: PBS +1% FBS +0.1% NaN3]
[Cell fixation: 2% formaldehyde solution]. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD45RO are readily searchable from our website. Different antibodies against the same target such as CD45RO may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.