anti-CD46 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD46; anti-CD46 antibody;
The CD46 cd46 (Catalog #MBS200297) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The CD46 antibody reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD46 can be used in a range of immunoassay formats including, but not limited to, ELISA (EIA), Western Blot (WB), Flow Cytometry (FC/FACS), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunohistochemistry (IHC).Recommended starting dilution is 1:1000. Researchers should empirically determine the suitability of the CD46 cd46 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD46 cd46 product has the following accession number(s) (GI #27502405) (NCBI Accession #NP_758860) (Uniprot Accession #P15529). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD46 antibody with the following immunoassay(s):
Western Blot (WB) (The cell lysates of Ramos, HeLa and A549 (40ug) were resolved by SDS-PAGE, transferred to PVDF membrane and probed with anti-human CD46 antibody (1:1000). Proteins were visualized using a goat anti-mouse secondary antibody conjugated to HRP and an ECL detection system.)
Immunofluorescence (IF) (ICC/IF analysis of CD46 in HeLa cells. The cell was stained with ATGA0326 (1:100). The secondary antibody (green) was used Alexa Fluor 488. DAPI was stained the cell nucleus (blue).)
Membrane Cofactor Protein (CD46) is a type 1 membrane protein that plays an important inhibitory role in the complement system. CD46 regulates the complement cascade by inhibiting C3b and C4b deposited on self tissue. This protein is a cofactor that binds to C3b and C4b, allowing their degradation by a plasma serine protease called factor I. This function resides in the complement control protein repeats (CCPs), with CCP1-4 essential for regulation. CD46 is implicated in the development and/or progression of selected cancer types.
Antigen Species: Human
Immunogen: Recombinant human CD46 (35-313aa) purified from E. coli. Clone Note: Anti-human CD46 mAb, clone AT2G9, is derived from hybridization of mouse F0 myeloma cells with spleen cells from BALB/c mice immunized with a recombinant human CD46 protein. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD46 are readily searchable from our website. Different antibodies against the same target such as CD46 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.