anti-CD48 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD48; anti-CD48 antibody;
The CD48 cd48 (Catalog #MBS834029) is an Antibody produced from Hamster and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD48 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).FC: 20 ul (0.5 ug)/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. Researchers should empirically determine the suitability of the CD48 cd48 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD48 cd48 product has the following accession number(s) (GI #365733590) (NCBI Accession #NP_001242959.1) (Uniprot Accession #P09326). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD48 antibody (FITC) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of BALB/c splenocytes with 0.25 ug of Armenian Hamster IgG Isotype Control (FITC) (blue histogram) or 0.25 ug of CD48 antibody (FITC) (purple histogram). Total viable cells were used for analysis.)
Armenian Hamster monoclonal CD48 antibody (FITC).
Biological Significance: The HM48-1 monoclonal antibody reacts with the mouse CD48 antigen also known as BCM1, Blast-1, and OX-45. CD48, a member of the SLAM family and Ig superfamily, is a 45 kDa GPI-linked glycoprotein expressed on the majority of hematopoietic cells. Recent publications have reported differential expression of members of the SLAM family including CD48, CD150, and CD244 among functionally distinct bone marrow hematopoietic progenitors providing an useful tool for prediction of the primitiveness of hematopoietic progenitors based on the expression of these SLAM family members. Hematopoietic stem cells are highly purified as CD150CD244CD48 cells while non-self-renewing multipotent hematopoietic progenitors are CD244CD150CD48 and the most restricted progenitors are CD48CD244CD150.CD48 plays a critical role in adhesion and T cell activation. In the mouse, the primary counter-receptors for CD48 are CD2 and CD244. HM48-1 is reported to modulate in vitro and in vivo CD48 functions including blocking the CD48/CD2 and CD48/CD244 interactions, inhibiting the proliferative response of mitogen-activated spleen cells, providing a costimulation signal for T cells activated in vitro through their TCR, and prolonging cardiac allograft survival in vivo. Tag/Conjugate: FITC. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD48 are readily searchable from our website. Different antibodies against the same target such as CD48 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.