|The CD49b cd49b (Catalog #MBS666083) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD49b-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD49b can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FX. Researchers should empirically determine the suitability of the CD49b cd49b for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD49b cd49b product has the following accession number(s) (GI #303387045) (NCBI Accession #ADM15550.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD49b-Purified (with Antibiotics) with the following immunoassay(s):
Information: Human CD49b is an integrin alpha 2 subunit that forms a heterodimer with beta 1 integrin and functions as an adhesion molecule. Antibody HAS6 recognizes the alpha 2 integrin subunit.
Performance: Five x 105 cultured UM-SCC (squamous cell carcinoma) cells were harvested by trypsinization, washed and preincubated 5 minutes with 20 ul of 250 ug/ml human IgG (To block nonspecific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD49b antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.29 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD49b
Immunogen: Human cultured keratinocytes. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD49b are readily searchable from our website. Different antibodies against the same target such as CD49b may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.