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anti-CD49d antibody product blog

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Posted on 2015-08-27 04:05:07 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD49d; anti-CD49d antibody;
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The CD49d n/a (Catalog #MBS666007) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD49d-Purified Preservative Free reacts with Human, Dog and may cross-react with other species as described in the data sheet. MyBioSource\'s CD49d can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FX. Researchers should empirically determine the suitability of the CD49d n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:


Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD49d-Purified Preservative Free with the following immunoassay(s):
Testing Data
Testing Data CD49d.

Information: Human CD49d molecules are integrin alpha4 chains that are expressed as heterodimers with CD29 (beta1 integrin) or (beta7 integrin) and function as adhesion receptors. CD49d/CD29 is mainly expressed on thymocytes and B cells with increased expression on activated T cells. The ligands for CD49d/CD29 include VCAM-1 and fibronectin. Antibody BU49 recognizes the CD49d molecule of about 145 kd.

Performance: Five x 105 cultured HPB-MLT human tumor cells were washed incubated 45 minutes on ice with 80 ul of anti-CD49d antibody diluted to 5 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.52 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin. Product was 0.2 um filtered and vialed under aseptic conditions.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl.
Immunogen: Human peripheral myeloma cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD49d are readily searchable from our website. Different antibodies against the same target such as CD49d may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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