|The CD49f n/a (Catalog #MBS666247) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD49f-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD49f can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), Western Blot (WB), Immunohistochemistry (IHC). Researchers should empirically determine the suitability of the CD49f n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD49f-Purified (with Antibiotics) with the following immunoassay(s):
Information: Human CD49f molecules are integrin alpha6 chains that are expressed as heterodimers with CD29 (beta1 integrin) or CD104 (beta4 integrin) and function as adhesion receptors. CD49f is expressed on platelets, monocytes, T lymphocytes and thymoctyes. The ligands for CD49f/CD29 include laminin and invasion. CD49f/CD29 has been shown to associate with CD63.
Performance: Five x 105 cultured Raji human tumor cells were washed and incubated 45 minutes on ice with 80 ml of anti-CD49f antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.78 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD49f
Immunogen: UM-UC-9, human bladder cancer cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD49f are readily searchable from our website. Different antibodies against the same target such as CD49f may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.