|The CD49f n/a (Catalog #MBS833226) is an Antibody produced from Rat and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD49f can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS).
FC: <= 1 ug/test
A test is defined as the amount (ug) of antibody that will stain a cell sample in a final volume of 100 ul. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest. Researchers should empirically determine the suitability of the CD49f n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the CD49f antibody (biotin) with the following immunoassay(s):
Flow Cytometry (FC/FACS) (Staining of normal human peripheral blood cells with 0.25 ug of Rat IgG2a kappa Isotype Control (biotin) (open histogram) or 0.125 ug of CD49f antibody (biotin) (filled histogram) followed by Streptavidin (PE). Cells in the lymphocyte gate were used for analysis.)
Rat monoclonal CD49f antibody (biotin).
Biological Significance: The GoH3 monoclonal antibody reacts with mouse and human CD49f, also known as integrin alpha 6, very late activation antigen 6, and platelet gpIc. CD49f is a 120 kD transmembrane protein. CD49f associates with CD29, the integrin beta 1 chain, to form the VLA-6 complex CD49f also associates with CD104, the integrin beta 4 chain, to form the alpha 6 beta 4 complex. CD49f is expressed primarily on T cells, monocytes, platelets, epithelial and endothelial cells. CD49f expression has also been found on germinal center B cells. The GoH3 antibody is cross-reactive to integrin alpha 6 on human, mouse and bovine cells. This antibody has also been reported to have functional activity in blocking the binding of integrin alpha 6 to laminin. Tag/Conjugate: Biotin. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD49f are readily searchable from our website. Different antibodies against the same target such as CD49f may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.