|The CD51 n/a (Catalog #MBS666538) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD51-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD51 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD51 n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
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Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD51-Purified (with Antibiotics) with the following immunoassay(s):
Information: Human CD51 is an integrin alpha v chain which can associate with five different integrin beta chains to form various adhesion molecules. The alpha v beta 3 integrin complex binds vitronectin at the RGD sequence and also can bind to fibrinogen, von Willebrand factor, thrombospondin, fibronectin, osteopontin and collagen. Antibody P2W7 recognizes the alpha v molecule of 160 kd.
Performance: Five x 105 cultured UM-SCC (squamous cell carcinoma) per tube were harvested by trypsinization, washed and preincubated 5 minutes with 20ul of 250ug/ml human IgG (to block nonspecific binding), after which they were incubated 45 minutes on ice with 80 ul of anti-CD51 antibody at 10 ug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 1.32 log10 fluorescent units when compared to a Mouse IgG1 negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD51
Immunogen: Human ocular melanoma cell line (V+B2). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD51 are readily searchable from our website. Different antibodies against the same target such as CD51 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.