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anti-CD58 antibody product blog

Posted on 2016-01-16 05:31:47 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD58; anti-CD58 antibody;
The CD58 cd58 (Catalog #MBS246025) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The Mouse Monoclonal [clone 2D11-B10] (IgG2a,k) to Human CD58 reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD58 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC - Paraffin), Immunofluorescence (IF), Western Blot (WB), ELISA (EIA).
ELISA, IF (10 ug/ml), IHC-P (5 ug/ml), WB (1:500 - 1:1000)
Usage: Immunohistochemistry: Formalin-fixed paraffin-embedded sections, Formalin/PFA-fixed paraffin-embedded sections. Sandwich ELISA: Recombinant protein. Western Blot: Cell lysate, Recombinant protein. Researchers should empirically determine the suitability of the CD58 cd58 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD58 cd58 product has the following accession number(s) (GI #4502677) (NCBI Accession #NP_001770.1) (Uniprot Accession #P19256). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the Mouse Monoclonal [clone 2D11-B10] (IgG2a,k) to Human CD58 with the following immunoassay(s):
Immunohistochemistry (IHC) (Anti-CD58 antibody IHC of human thymus. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)
Immunohistochemistry (IHC) CD58.

Immunofluorescence (IF) (Immunofluorescence of monoclonal antibody to CD58 on HeLa cell. [antibody concentration 10 ug/ml])
Immunofluorescence (IF) CD58.

Western Blot (WB) (CD58 monoclonal antibody (M01), clone 2D11-B10 Western blot of CD58 expression in Jurkat.)
Western Blot (WB) CD58.

ELISA (EIA) (Detection limit for recombinant GST tagged CD58 is approximately 0.3 ng/ml as a capture antibody.)

Target Species: Human
Immunogen Description: CD58 (AAH05930, 1 a.a. ~ 241 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. Immunogen: CD58 antibody was raised against cD58 (AAH05930, 1 a.a. ~ 241 a.a) full length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD58 are readily searchable from our website. Different antibodies against the same target such as CD58 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. CD58 also interacts with the following gene(s): CD2, CD2AP, CD40, CD86, ICAM1, IFNA17, ITGAL.
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