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anti-CD62P antibody product blog

Posted on 2016-02-20 17:53:30 by mybiosource_staff
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Tags: Antibody; Monoclonal Antibody; CD62P; anti-CD62P antibody;
The CD62P n/a (Catalog #MBS666558) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD62P-Purified (with Antibiotics) reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD62P can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS), FX. Researchers should empirically determine the suitability of the CD62P n/a for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.

The CD62P n/a product has the following accession number(s) (GI #327239666) (NCBI Accession #AEA39677.1). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.

To buy or view more detailed product information and pricing, please click on the technical datasheet page below:

Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD62P-Purified (with Antibiotics) with the following immunoassay(s):
Testing Data
Testing Data CD62P.

Information: Human CD62P appears on the surface of activated endothelial cells or platelets and likely is involved with regulating the recruitment of leukocytes to areas of inflammation or infection (1, 2). Antibody G1 recognizes the protease sensitive lectin epitope of CD62P (1). Antibody G1 blocks the activated endothelium or platelet-neutrophil interaction (3, 4).

Performance: Platelet rich plasma (PRP) from heparinized blood was activated with 1 U/ml Thrombin in the presence of Citrate for 10 minutes. Ten ul of PRP per tube were fixed in 1% buffered formaldehyde, washed twice and pre incubated 5 minutes with 20 ul of human IgG at 250 ug/ml (to block non specific binding) after which they were incubated 45 minutes on ice with 80 ul of anti-CD62P antibody at 10 ug/ml. Tubes were washed and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed again, re fixed and analyzed by FACS. A net 84% of the gated population stained positive with a mean shift of 1.83 log10 fluorescent units when compared to a Mouse IgG1 negative control. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE with less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Group Header: anti-human CD62P
Immunogen: Purified human platelet GMP-140 (CD62P). In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD62P are readily searchable from our website. Different antibodies against the same target such as CD62P may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results.
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