anti-CD69 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD69; anti-CD69 antibody;
The CD69 cd69 (Catalog #MBS666339) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The anti-human CD69-Purified Preservative Free reacts with Human and may cross-react with other species as described in the data sheet. MyBioSource\'s CD69 can be used in a range of immunoassay formats including, but not limited to, Flow Cytometry (FC/FACS). Researchers should empirically determine the suitability of the CD69 cd69 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.The CD69 cd69 product has the following accession number(s) (GI #793838) (NCBI Accession #CAA86225.1) (Uniprot Accession #Q6LAB3). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the anti-human CD69-Purified Preservative Free with the following immunoassay(s):
Testing Data
Information: Human CD69 is expressed by platelets and mature thymocytes and appears rapidly after activation on T cells, B cells and NK cells. This pattern of cellular CD69 expression has suggested a lymphocyte activation role. Functional studies with antibodies to CD69 also support an activation role for CD69. Antibody HP-4B3 recognizes the dimeric CD69 molecule of 60 kd.
Performance: Five x 105 cultured HEL cells were washed and incubated 45 minutes on ice with 80 mul of anti-CD69 antibody at 5 mug/ml. Cells were washed twice and incubated with 2 degree reagent Goat anti-Mouse IgG/FITC, after which they were washed three times, fixed and analyzed by FACS. Cells stained positive with a mean shift of 0.44 log10 fluorescent units when compared to a Mouse IgG2a negative control at a similar concentration. Production: Antibody was Protein A purified from (low FBS containing) tissue culture supernatant. Purity was >95% Immunoglobulin by SDS-PAGE and contains less than 1% Bovine Immunoglobulin.
Buffer: 50 mM Sodium Phosphate pH 7.5, 100 mM Potassium Chloride, 150mM NaCl, 0.5 mg/ml Gentamicin Sulfate (as a preservative).
Immunogen: IL-2 activated human NK cells. In general, we may offer more than one antibody to a given target to enable options for the researcher. Available antibodies recognizing CD69 are readily searchable from our website. Different antibodies against the same target such as CD69 may be optimized or tested for different applications and species. This enables researchers to select the option that may be best for their model system, to screen more than antibody to determine which one may be best for their model system, as well as to use more than one antibody to follow up on and validate their results. Blood, Bone Marrow, Intestine, Kidney, Liver, Lung, Lymph Node, Spleen, Thymus, Vascular tissues are correlated with this protein.