anti-CD70 antibody product blog
Tags: Antibody; Monoclonal Antibody; CD70; anti-CD70 antibody;
The CD70 cd70 (Catalog #MBS214279) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. MyBioSource\'s CD70 can be used in a range of immunoassay formats including, but not limited to, Immunohistology Frozen, Flow cytometry (FC/FACS).Flow Cytometry: Use 10ul of the suggested working dilution to label 106 cells in 100ul.
Flow Cytometry: Minimum Dilution: 1/50; Maximum Dilution: 1/200. Researchers should empirically determine the suitability of the CD70 cd70 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process.
The CD70 cd70 product has the following accession number(s) (GI #4507605) (NCBI Accession #NP_001243.1) (Uniprot Accession #P32970). Researchers may be interested in using Bioinformatics databases such as those available at The National Center for Biotechnology Information (NCBI) website for more information about accession numbers and the proteins they represent. Even researchers unfamiliar with bioinformatics databases will find the NCBI databases to be quite user friendly and useful.
To buy or view more detailed product information and pricing, please click on the technical datasheet page below:
Please refer to the product datasheet for known applications of a given antibody. We\'ve tested the MOUSE ANTI HUMAN CD70 with the following immunoassay(s):
Testing Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69, red in An and Mouse anti Human CD21 antibody, clone LB21 (MBS212051), green in B. C is the merged image with nuclei counterstained blue using DAPI. High power)
Testing Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 followed by the Histar setection system. Low power)
Testing Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 followed by the Histar setection system. Medium power)
Testing Data (Immunofluorescence staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69, red in An and Mouse anti Human CD21 antibody, clone LB21 (MBS212051), green in B. C is the merged image with nuclei counterstained blue using DAPI. Low power)
Testing Data (Immunoperoxidase staining of a human tonsil cryosection with Mouse anti Human CD70 antibody, clone Bu69 followed by the Histar setection system. High power)
Mouse anti Human CD70 antibody, clone Bu69 recognises human CD70, also known as CD27 ligand (CD27-L) or Tumor necrosis factor ligand superfamily member 7. CD70 is a 193 amino acid ~29kDa single pass type II transmembrane glycoprotein belonging to the tumour necrosis factor (TNF) family (UniProt: P32970). CD70 is expressed on a small number of activated T-cells and the majority of activated B-cells (Bowman et al. 1994). Interaction of CD70 with its receptor, CD27, is thought to play an important role in lymphocyte activation, proliferation, survival and differentiation (Hintzen et al. 1994). Mouse anti Human CD70 antibody, clone Bu69 has been used for the inhibition of neural stem cell induced apoptosis of allogeneic T cells in vitro (Lee et al. 2013).
Perservative Stabilisers: 0.09% Sodium Azide
Preparation: Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant.